Annoying vibrations in grandstand structures have been receiving more attention due to the increasing slenderness of the architectural components and the complexity of the crowd loading for engineers. The vibration serviceability checks under these conditions become a challenge in the design and operation stages. Regarding human comfort, excessive vibrations due to occupant activities may affect comfort and/or cause panic, especially for passive occupants who do not participate in generating excitations. Although durations of excessive vibrations have been considered as one of the most important factors affecting occupant comfort, incorporating the vibration duration in the occupant comfort analysis has not been addressed yet. In addition, the currently available approaches using raw acceleration, weighted RMS acceleration, vibration dose values (VDV), and so on may not always be sufficient for serviceability assessment due to the lack of guided procedure for calculating the integration time and implementing the duration of vibration into the process. Therefore this study proposes a new parameter and framework for assessing human comfort which incorporates the duration of vibration with conventional data processing. The aim is to better examine vibration levels and the corresponding occupant response focusing on grandstand structures. A new parameter, the area of RMS (ARMS), is introduced using the running RMS values of acceleration weighted by the frequency weighting functions. Furthermore, perception ranges for human comfort levels based on the proposed parameter are presented. The experimental study reveals that the proposed framework can successfully address the impact of duration time on determining the levels of vibrations and comfort using the proposed parameter.